Доктор Данте Зарленга Департамент сельского хозяйства сша, Лаборатория Паразитологии животных Бэлтсвилль, США



Дата03.07.2017
өлшемі445 b.



Taeniosis Dante Zarlenga Ph.D US Department of Agriculture Agricultural Research Service Animal Parasitic Diseases & Bovine Functional Genomics Labs Beltsville, MD USA Тениозы доктор Данте Зарленга Департамент сельского хозяйства США, Лаборатория Паразитологии животных Бэлтсвилль, США

















1) Cysticercosis:

  • 1) Cysticercosis:

  • Цистицеркоз:

  • Serology: Серологический

  • EITB - enzyme-linked immunoelectrotransfer blot; 100% sensitive when 2 or more cysts present; 100% specific. Commercialized gold standard; expensive

  • EITB – метод иммуноэлектроблотинга с ферментативным усилением; чувствительность 100% при наличии 2 и более цист; специфичность 100%. Является дорогостоящим.

  • ELISA- less sensitive, but improving.

  • ELISA – менее чувствительный, но усовершенствуется

  • Circulating Antigen - 85% sensitive; some specificity

  • problems.

  • Антигенный – чувствительность 85%; проблемы, связанные со специфичностью.



  • 2) Neurocysticercosis

  • Medical Imaging (CT and MRI) - greatly improved diagnosis in a clinical setting.

  • Serology - EITB, ELISA

  • 2) Нейроцистицеркоз

  • Рентгенография (CT и MRI) – позволяет производить точную диагностику в клинических условиях.

  • Серологические методы – EITB, ELISA











Administering Oxfendazole Введение оксфендазола



I. In transmission of eggs from people to pigs

  • I. In transmission of eggs from people to pigs

  • - Rearing Free-range pigs

  • - Accessibility of pigs to human feces

  • - Involvement of human carriers in pig

  • rearing

  • - Use of “night soil”, sewage effluent or

  • sludge in farming



2. In transmission of cysticerci to humans

  • 2. In transmission of cysticerci to humans

  • - Lack of adequate meat inspection

  • - Clandestine pig marketing

  • - Cultural preferences for raw or lightly cooked pork



Improve inspection procedures

  • Improve inspection procedures

  • Improve animal rearing (reduce exposure to human waste)

  • Mass drug treat pigs at risk.

  • Develop animal vaccines



Vaccines based mainly on oncosphere antigens.

  • Vaccines based mainly on oncosphere antigens.

  • Recombinant Peptide Vaccines: Highly protective (near 100%) in trials, but no reliable field data. Commercially viable?

  • DNA+Peptide Vaccine: Initial results promising. Cost and commercialization problematical.













USDA, ARS, ANRI Animal Parasitic Diseases Laboratory

  • Trichinella spiralis

  • A stigma for U.S. pork both domestically and internationally

  • Является проблемой для качества свинины как в США, так и за рубежом

  • Nematode parasite of nearly all mammals; global distribution

  • Нематода паразитирует почти у всех млекопитающих; распространена по всему миру









Reported Trichinellosis cases, United States, 1947-2001 Зарегистрированные случаи трихинеллеза в США, 1947-2001



Reported Trichinellosis cases, United States, 1997-2001 Зарегистрированные случаи трихинеллеза в США , 1997-2001









Monitoring Programs for T. spiralis in US pig population Программы контроля T. spiralis у свиней в США

  • - Support of export program for EU and Russia

  • - An export market inspection program was established

  • - USDA trains meat inspectors each year

  • - USDA supports inspection programs in slaughter plants

  • - Export market of pork and horsemeat to EU and Russia is preserved



Monitoring Programs for T. spiralis in US pig population Программы контроля T. spiralis у свиней в США

  • - Trichinae certification program

  • - On-farm certification program to assure food safety

  • - Program audits farms for good production practices

  • - Program limits risk of exposure of pigs to infection on farms supplying the packing plants.





TRICHINAE CERTIFICATION PROGRAM

  • 123 pork producers in USA now participating in a 5 year pilot study

  • Periodic serology testing of certified pigs at packing plant

  • Viable alternative to individual carcass testing (EU)

  • Possible replacement for Export Program








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